By: OLUFAYO M. O.; ADEYANJU A. A.;
Abstract:
This study examined the impact of Azardirachta indica leaves extract on blood composition and flesh of H. bidorsalis under laboratory conditions over a 96 h exposure period. Concentrations of A.indica leaves extract used were 0.00 (control),5.00, 10.00, 15.00, 20.00 and 25.00mg/l. A total of one hundred and twenty juveniles of H.bidorsalis (wt.15.91 + 0.96g) were selected randomly at 10 fish per aquarium for the experiment with duplicate of each treatment.The extract led to increase in the opercular ventilation rates, erractic swimming, respiration distress in the test fish at the highest concentrations (20 and 25 mg/l). With the exception of PCV, Hb, RBC, the values of all the parameters in the control were lower than those in the treatments. WBC, MCHC,MCH and MCV values in the treatments increased with increase in concentrations of neem leaves extract. There is significant difference in the haematological parameters between in the control and the treatment levels (P<0.05). There was a significant reduction in the neutrophils of the fish exposed to 10.00, 15.00, and 25.00 mg/l of the extract. Significantly (P<0.05) low fat and low protein were observed while there was a significant increase in %Ash as the concentrations of A. indica leaves extract increased.
Keywords: Neem leaves, Azadirachta indica , Heterobranchus bidorsalis,Haematology ,Leaves extract.
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